Application
Immunofluorescence analysis of methanol-fixed HeLa, using PRPS1 antibody at 1:200 dilution.Application
Sample (30 µg of whole cell lysate) A: Molt-4 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using PRPS1 antibody at 1:500 dilution.| Product Name | PRPS1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PRPS1 (phosphoribosyl pyrophosphate synthetase 1)This gene encodes an enzyme that catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for purine metabolism and nucleotide biosynthesis. Defects in this gene are a cause of phosphoribosylpyrophosphate synthetase superactivity, Charcot-Marie-Tooth disease X-linked recessive type 5 and Arts Syndrome. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PRPS1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Rhesus Monkey, BovineMouse (100%), Rat (100%), Xenopus laevis (95%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ARTS antibody CMTX5 antibody DFN2 antibody DFNX1 antibody KIAA0967 antibody PPRibP antibody PRSI antibody PRPS1 antibody dJ1070B1.2 (phosphoribosyl pyrophosphate synthetase 1) antibody "deafness X-linked 2 perceptive congenital antibody" "deaf |
|---|---|
| Molecular Weight(MW) | 35 kDa (note) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using PRPS1 antibody at 1:200 dilution.
Application
Sample (30 µg of whole cell lysate) A: Molt-4 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using PRPS1 antibody at 1:500 dilution.| Positive Control | 293T , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
