Application
Immunocytochemistry of APG7 in L1210 cells with APG7 antibody at 10 μg/mL.Application
Immunofluorescence of APG7 in L1210 cells with APG7 antibody at 10 μg/mL.Application
Western blot analysis of APG7 in L1210 cell lysate with APG7 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.| Product Name | APG7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APG7 Antibody: GSA7, APG7L, APG7-LIKE, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, APG7-like |
| Product description | APG7 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906. |
| Immunogen | APG7 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human APG7. The immunogen is located within the first 50 amino acids of APG7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| APG7 antibody can be used for the detection of APG7 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATG7 |
|---|---|
| Alternative Names | APG7 Antibody: GSA7 APG7L APG7-LIKE Ubiquitin-like modifier-activating enzyme ATG7 ATG12-activating enzyme E1 ATG7 APG7-like |
| Molecular Weight(MW) | Predicted: 77 kDa Observed: 77 kDa |
| Sequence Similarities | 2) Cat. No. 17-204 - L1210 Cell Slide |
Application
Immunocytochemistry of APG7 in L1210 cells with APG7 antibody at 10 μg/mL.
Application
Immunofluorescence of APG7 in L1210 cells with APG7 antibody at 10 μg/mL.
Application
Western blot analysis of APG7 in L1210 cell lysate with APG7 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.| Positive Control | 1) Cat. No. 1284 - L1210 Cell Lysate |
|---|---|
| Application Notes | APG7 antibody can be used for the detection of APG7 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | APG7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | APG7 Antibody is supplied in PBS containing 0.02% sodium azide. |
