ApoA1 Antibody: Apolipoprotein A1 (ApoA1) is the major protein component of high density lipoprotein (HDL) in plasma, which is correlated with cardiovascular disease. ApoA1 is synthesized in the liver and small intestine and promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT), the enzyme responsible for the formation of most plasma cholesteryl esters. Defects in ApoA1 are associated with HDL deficiency, Tangier disease, and systemic non-neuropathic amyloidosis.1) Zhu X, Wu G, Zeng W, et al. Cysteine mutants of human apolipoprotein A-I: a study of secondary structural and functional properties. J. Lipid Res. 2005; 46:1303-11
Immunogen
ApoA1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ApoA1. The immunogen is located within the last 50 amino acids of ApoA1.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAWB
ApoA1 antibody can be used for detection of ApoA1 by Western blot at 1 μg/mL.:
Western blot analysis of ApoA1 in chicken liver tissue lysate with ApoA1 antibody at 1 μg/mL .
Positive Control
1) Chicken Liver Lysate
Application Notes
ApoA1 antibody can be used for detection of ApoA1 by Western blot at 1 μg/mL.:
Additional Information
Form
Liquid
Storage Instructions
ApoA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
ApoA1 Antibody is supplied in PBS containing 0.02% sodium azide.