Application
Western blot analysis of ApoA4 in human testis tissue lysate with ApoA4 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | ApoA4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ApoA4 Antibody: Apolipoprotein A-IV, Apolipoprotein A4, Apo-AIV |
| Product description | ApoA4 Antibody: Apolipoprotein A4 (also known as ApoA-IV) is a plasma protein that is an O-linked glycoprotein after proteolytic processing. ApoA4 is a major constituent of HDL and it also can be found on the surface of newly synthesized chylomicrons. The physiological function of ApoA4 is not completely identified so far, but evidence from several in vitro studies indicate that this apolipoprotein is involved in several steps of the reverse cholesterol transport pathway, which transports cholesterol from peripheral cells back to the liver.1) Karathanasis SK, Oettgen P, Haddad IA, et al. Structure, evolution, and polymorphisms of the human apolipoprotein A4 gene (APOA4). Proc. Natl. Acad. Sci. USA 1986; 83: 8457-61 |
| Immunogen | ApoA4 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ApoA4. The immunogen is located within amino acids 320 - 370 of ApoA4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ApoA4 antibody can be used for detection of ApoA4 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | ChickenHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APOA4 |
|---|---|
| Alternative Names | ApoA4 Antibody: Apolipoprotein A-IV Apolipoprotein A4 Apo-AIV |
| Molecular Weight(MW) | Predicted: 41 kDa Observed: 40 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig: (100%) |
| Entrez Gene | 337 |
|---|---|
| Protein Accession | NCBI accession No. AAA51744 |
Application
Western blot analysis of ApoA4 in human testis tissue lysate with ApoA4 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | ApoA4 antibody can be used for detection of ApoA4 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ApoA4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ApoA4 Antibody is supplied in PBS containing 0.02% sodium azide. |
