Application
Western blot analysis of APOBEC3B in rat small intestine tissue lysate with APOBEC3B antibody at 1 μg/ml.Application
Immunofluorescence of APOBEC3B in human small intestine tissue with APOBEC3B antibody at 20 μg/mL.Application
Immunohistochemistry of APOBEC3B in human small intestine tissue with APOBEC3B antibody at 5 μg/mL.| Product Name | APOBEC3B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APOBEC3B Antibody: A3B, ARP4, ARCD3, PHRBNL, APOBEC1L, bK150C2.2, DJ742C19.2Phorbolin-1-related protein, A3B |
| Product description | The Apolipoprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytosine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates (1). Similar to TRIM5? which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection (2,3). APOBEC3B has also been shown to be upregulated in several cancers; it is thought that APOBEC3B-catalyzed genomic uracil lesions are responsible for a large proportion of mutations in multiple distinct cancers (4).1) Jarmuz A, Chester A, Bayliss J, et al. An anthropoid-specific locus of Orphan C to U RNA-editing enzymes on chromosome 22. Genomics 2002; 79:285-96. |
| Immunogen | APOBEC3B antibody was raised against a 14 amino acid peptide near the amino terminus of human APOBEC3B. The immunogen is located within amino acids 20 - 70 of APOBEC3B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| APOBEC3B antibody can be used for detection of APOBEC3B by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APOBEC3B |
|---|---|
| Alternative Names | APOBEC3B Antibody: A3B ARP4 ARCD3 PHRBNL APOBEC1L bK150C2.2 DJ742C19.2Phorbolin-1-related protein A3B |
| Molecular Weight(MW) | Predicted: 42 kDa Observed: 48 kDa |
| Sequence Similarities | 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| Tissue Specificity | APOBEC3B antibody is human, mouse and rat reactive . At least three isoforms of APOBEC3B are known to exist; this antibody will detect all three. APOBEC3B antibody may cross-react with APOBEC3D. |
Application
Western blot analysis of APOBEC3B in rat small intestine tissue lysate with APOBEC3B antibody at 1 μg/ml.
Application
Immunofluorescence of APOBEC3B in human small intestine tissue with APOBEC3B antibody at 20 μg/mL.
Application
Immunohistochemistry of APOBEC3B in human small intestine tissue with APOBEC3B antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1468 - Rat Small Intestine Tissue Lysate |
|---|---|
| Application Notes | APOBEC3B antibody can be used for detection of APOBEC3B by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | APOBEC3B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | APOBEC3B Antibody is supplied in PBS containing 0.02% sodium azide. |
