Application
Immunohistochemical staining of human spleen using APOBEC3G antibody at 1 μg/mL.Application
Western blot analysis of APOBEC3G expression in Caco-2 cell lysate in the (A), absence and (B) presence of blocking peptide with APOBEC3G antibody at 5 μg/mL.| Product Name | APOBEC3G Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APOBEC3G Antibody: A3G, ARCD, ARP9, ARP-9, CEM15, CEM-15, MDS019, bK150C2.7, dJ494G10.1APOBEC-related cytidine deaminase, APOBEC-related protein |
| Product description | APOBEC3G Antibody: The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates. Similar to TRIM5α which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome. This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity.1) Jarmuz A, Chester A, Bayliss J, et al. An anthropoid-specific locus of Orphan C to U RNA-editing enzymes on chromosome 22. Genomics 2002; 79:285-96. |
| Immunogen | APOBEC3G antibody was raised against a synthetic peptide corresponding to 15 amino acids near the amino-terminus of human APOBEC3G. The immunogen is located within the first 50 amino acids of APOBEC3G. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| APOBEC3G antibody can be used for detection of APOBEC3G by Western blot at 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APOBEC3G |
|---|---|
| Alternative Names | APOBEC3G Antibody: A3G ARCD ARP9 ARP-9 CEM15 CEM-15 MDS019 bK150C2.7 dJ494G10.1APOBEC-related cytidine deaminase APOBEC-related protein |
| Sequence Similarities | 3) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | APOBEC3G antibody will also detect the APOBEC3F isoform. |
Application
Immunohistochemical staining of human spleen using APOBEC3G antibody at 1 μg/mL.
Application
Western blot analysis of APOBEC3G expression in Caco-2 cell lysate in the (A), absence and (B) presence of blocking peptide with APOBEC3G antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1223 - Caco-2 Cell Lysate |
|---|---|
| Application Notes | APOBEC3G antibody can be used for detection of APOBEC3G by Western blot at 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | APOBEC3G antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | APOBEC3G Antibody is supplied in PBS containing 0.02% sodium azide. |
