Application
APOL1 Antibody IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometricof MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the detected the APOL1-BH3 Domain protein (arrow).| Product Name | APOL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Apolipoprotein L1, Apolipoprotein L, Apo-L, ApoL, Apolipoprotein L-I, ApoL-I, APOL1, APOL |
| Product description | APOL1 is a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver.1) Li,Q., Clin. Chim. Acta 403 (1-2), 152-155 (2009) |
| Immunogen | This APOL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-171 amino acids from human APOL1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | APOL1 |
|---|---|
| Alternative Names | Apolipoprotein L1 Apolipoprotein L Apo-L ApoL Apolipoprotein L-I ApoL-I APOL1 APOL |
| Molecular Weight(MW) | 44 kDa |
Application
APOL1 Antibody IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometricof MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the detected the APOL1-BH3 Domain protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
