Application
Western blot analysis of AQP2 in A431 cell lysate with AQP2 antibody at 1 μg/ml.Application
Immunofluorescence of AQP2 in human kidney tissue with AQP2 antibody at 20 μg/mL.Application
Immunohistochemistry of AQP2 in human kidney tissue with AQP2 antibody at 5 μg/mL.| Product Name | AQP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AQP2 Antibody: AQP-CD, WCH-CD, Aquaporin-2, ADH water channel, AQP-2 |
| Product description | Aquaporins are membrane proteins that serve in the transfer of water and small solutes across cellular membranes. One such aquaporin, aquaporin-2 (AQP2) is located in the kidney collecting tubule and plays a critical role in water reabsorbtion (1). AQP2 is mainly localized in intracellular vesicles but upon stimulation with anti-diuretic hormone (ADH), AQP2 is translocated to the apical plasma membrane by exocytic fusion of AQP2-bearing vesicles (2). Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus (3).1) Denker BM, Smith BL, Kuhada FP, et al. Identification, purification, and partial characterization of a novel Mr 28,000 integral membrane protein from erythrocytes and renal tubules. J. Biol. Chem. 1988; 263:15634-42. |
| Immunogen | AQP2 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AQP2. The immunogen is located within the last 50 amino acids of AQP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| AQP2 antibody can be used for detection of AQP2 by Western blot at 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AQP2 |
|---|---|
| Alternative Names | AQP2 Antibody: AQP-CD WCH-CD Aquaporin-2 ADH water channel AQP-2 |
| Molecular Weight(MW) | Predicted: 30 kDa Observed: 28 kDa |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | AQP2 antibody is human, mouse and rat reactive. This antibody is predicted to not cross-react with other members of the aquaporin protein family. |
Application
Western blot analysis of AQP2 in A431 cell lysate with AQP2 antibody at 1 μg/ml.
Application
Immunofluorescence of AQP2 in human kidney tissue with AQP2 antibody at 20 μg/mL.
Application
Immunohistochemistry of AQP2 in human kidney tissue with AQP2 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1202 - A431 Cell Lysate |
|---|---|
| Application Notes | AQP2 antibody can be used for detection of AQP2 by Western blot at 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | AQP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | AQP2 antibody is supplied in PBS containing 0.02% sodium azide. |
