Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ARHE antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:2000 dilution Lane 1: mouse brain lysate Lane 2: rat brain lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of ARHE Antibody (Cat. 3AP7751c) in mouse brain tissue lysates (35ug/lane)| Product Name | ARHE Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Rho-related GTP-binding protein RhoE, Protein MemB, Rho family GTPase 3, Rho-related GTP-binding protein Rho8, Rnd3, RND3, ARHE, RHO8, RHOE |
| Product description | Members of the Rho family of Ras-related GTPases, such as ARHE, regulate the organization of the actin cytoskeleton in response to extracellular growth factors. Like Ras (MIM 190020), Rho family members appear to cycle between an inactive GDP-bound form and an active GTP-bound form. Three major regulators of Rho activity have been identified: RhoGDIs, which interact with the GDP-bound Rho proteins to keep them in a resting complex (see MIM 601925); GEFs, which promote GDP/GTP exchange leading to activation of Rho proteins (see MIM 601855); and GAPs, which stimulate GTP hydrolysis and return the activated Rho protein to its inactive form (see MIM 602680) (Nobes et al., 1998 [PubMed 9531558]).1) Pinner,S., Nat. Cell Biol. 10 (2), 127-137 (2008) |
| Immunogen | This ARHE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-165 amino acids from the Central region of human ARHE. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Symbol | RND3 |
|---|---|
| Alternative Names | Rho-related GTP-binding protein RhoE Protein MemB Rho family GTPase 3 Rho-related GTP-binding protein Rho8 Rnd3 RND3 ARHE RHO8 RHOE |
| Molecular Weight(MW) | 27 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ARHE antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:2000 dilution Lane 1: mouse brain lysate Lane 2: rat brain lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of ARHE Antibody (Cat. 3AP7751c) in mouse brain tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
