Application
staining Peroxiredoxin 1 in human TSU-Pr1 cells by Immunocytochemistry.Application
Western blot analysis of Peroxiredoxin was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a rabbit polyclonal antibody recognizing Peroxiredoxin at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using ECL substrate.| Product Name | PRX I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Peroxiredoxin 1Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, particularly amino-terminal region containing active site cysteine residue, and the thiolspecific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx6 belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20-30 kilodalton in molecular size and vary in subcellular localization: Prx1, 2, and 6 in cytosol, Prx3 in mitochondria, Prx 4 in ER and secretion, Prx 5 showing complicated distribution including peroxisome, mitochondria and cytosol. |
| Immunogen | Synthetic peptide: LVSDPKRTIAQD, corresponding to amino acids 103-114 of Human Peroxiredoxin 1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| ICC/IF: 1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MSP23 NKEFA PAG PAGA PAGB PRX1 PRXI TDPX2 |
|---|---|
| Target | Peroxiredoxin 1 |
| Tissue Specificity | This antibody detects Peroxiredoxin 1 protein in human samples. The antibody is specific for Peroxiredoxin 1 and shows no cross reactivity with other Prx isoforms. |
| Cellular Localization | Cytoplasmic |
Application
staining Peroxiredoxin 1 in human TSU-Pr1 cells by Immunocytochemistry.
Application
Western blot analysis of Peroxiredoxin was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a rabbit polyclonal antibody recognizing Peroxiredoxin at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using ECL substrate.| Positive Control | TSU Pr1 cells for cell staining, human PC3 cell lysate for western blotting. |
|---|---|
| Application Notes | ICC/IF: 1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: Whole serum |
