Application
Western blot analysis of ARL15 in K562 cell lysate with ARL15 antibody at 1 μg/mL.Application
Immunohistochemistry of ARL15 in human spleen tissue with ARL15 antibody at 5 μg/mL.| Product Name | ARL15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARL15 Antibody: ARFRP2, ARFRP2, ADP-ribosylation factor-like protein 15, ADP-ribosylation factor-related protein 2, ARF-related protein 2 |
| Product description | ARL15 Antibody: ADP-ribosylation factors (ARFs) are highly conserved guanine nucleotide binding proteins that enhance the ADP-ribosyltransferase activity of cholera toxin. ARFs are important in eukaryotic vesicular trafficking pathways and they play an essential role in the activation of phospholipase D (PC-PLD). ARL15 (ADP-ribosylation factor-like 15), is a member of the ARF family of the Ras superfamily of small GTPases that are known to be involved in multiple regulatory pathways altered in human carcinogenesis. Other members of this protein family include ARL11, thought to be a tumor suppressor and may play a role in the regulation of apoptosis, ARL2 which plays an important role in microtubule dynamics and cell cycle progression, and ARL4, which may play a role in neurogenesis during embryonic development. The gene encoding ARL15 maps to chromosome 5p15 in humans. At least three isoforms of ARL15 are known to exist.1) Pasqualato S, Renault L, and Cherfils J. ARF, ARL, ARP and SAR proteins: a family of GTP-binding proteins with a structural device for ‘front-back'communication. EMBO Rep.2002; 3: 1035-41. |
| Immunogen | ARL15 antibody was raised against a 17 amino acid synthetic peptide near the center of human ARL15. The immunogen is located within amino acids 130 - 180 of ARL15. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| ARL15 antibody can be used for detection of Arl15 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARL15 |
|---|---|
| Alternative Names | ARL15 Antibody: ARFRP2 ARFRP2 ADP-ribosylation factor-like protein 15 ADP-ribosylation factor-related protein 2 ARF-related protein 2 |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Western blot analysis of ARL15 in K562 cell lysate with ARL15 antibody at 1 μg/mL.
Application
Immunohistochemistry of ARL15 in human spleen tissue with ARL15 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | ARL15 antibody can be used for detection of Arl15 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ARL15 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ARL15 Antibody is supplied in PBS containing 0.02% sodium azide. |
