Application
Western Blot at 1:1000 dilution + human uterus lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of anti-CYP19A1 Pab in Jurkat cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human placenta tissue reacted with CYP19A1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | Aromatase Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Aromatase, CYPXIX, Cytochrome P-450AROM, Cytochrome P450 19A1, Estrogen synthase, CYP19A1, ARO1, CYAR, CYP19 |
| Product description | CYP19A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis, three successive hydroxylations of the A ring of androgens. Mutations in the gene encoding CYP19A1 can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation.1) Xita,N.,Eur. J. Endocrinol. 158 (6), 861-865 (2008) |
| Immunogen | This Aromatase (CYP19A1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 453-485 amino acids from the C-terminal region of human Aromatase (CYP19A1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CYP19A1 |
|---|---|
| Alternative Names | Aromatase CYPXIX Cytochrome P-450AROM Cytochrome P450 19A1 Estrogen synthase CYP19A1 ARO1 CYAR CYP19 |
| Molecular Weight(MW) | 58 kDa |
Application
Western Blot at 1:1000 dilution + human uterus lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of anti-CYP19A1 Pab in Jurkat cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human placenta tissue reacted with CYP19A1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
