Application
Western blot analysis of ARR3 in EL4 cell lysate with ARR3 antibody at (A) 0.5 and (B) 1 μg/ml.| Product Name | ARR3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARR3 Antibody: ARRX, ARRX, CAR, Arrestin-C, Cone arrestin, C-arrestin |
| Product description | Arrestin 3 (ARR3) belongs to the Arrestin family of proteins that function as negative regulators of G protein-coupled receptor (GPCR) signaling (1,2). ARR3 binds the c-Jun N-terminal kinase 3 (JNK3) and scaffolds the apoptosis signal-regulating kinase 1 (ASK1)-MAPK kinase4-JNK3 cascade in a receptor-independent fashion, promoting JNK3 phosphorylation (3,4). ARR3 also mediates the internalization of the C-C chemokine receptor CCR7 upon the binding of its ligand CCL19, but not CCL21, suggesting that it helps mediate CCR7 receptor desensitization (5).1) Murakami A, Yajima T, Sakuma H, et al. X-arrestin: a new retinal arrestin mapping to the X chromosome. FEBS Lett. 1993; 334:203-9. |
| Immunogen | ARR3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ARR3. The immunogen is located within the last 50 amino acids of ARR3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ARR3 antibody can be used for detection of ARR3 by Western blot at 0.5 - 1 μg/ml.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARR3 |
|---|---|
| Alternative Names | ARR3 Antibody: ARRX ARRX CAR Arrestin-C Cone arrestin C-arrestin |
| Molecular Weight(MW) | Predicted: 43 kDa Observed: 47 kDa |
| Tissue Specificity | ARR3 antibody is human, mouse and rat reactive. At least three isoforms of ARR3 are known to exist. This antibody is predicted to not cross-react with other members of the arrestin protein family. |
Application
Western blot analysis of ARR3 in EL4 cell lysate with ARR3 antibody at (A) 0.5 and (B) 1 μg/ml.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | ARR3 antibody can be used for detection of ARR3 by Western blot at 0.5 - 1 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ARR3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ARR3 antibody is supplied in PBS containing 0.02% sodium azide. |
