Application
Peroxiredoxin 3 antibody [N1C3] detects Peroxiredoxin 3 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: Peroxiredoxin 3 protein stained by Peroxiredoxin 3 antibody [N1C3] diluted at 1:1000. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded human lung cancer, using Peroxiredoxin 3 antibody at 1:100 dilution.Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 C: Molt-4 D: Raji 12% SDS PAGE diluted at 1:1000| Product Name | PRX III antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Peroxiredoxin 3 (peroxiredoxin 3)This gene encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Peroxiredoxin 3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, Rhesus Monkey, BovineRat (85%), Dog (91%), Pig (90%), Rhesus Monkey (97%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AOP-1 antibody AOP1 antibody MER5 antibody MGC104387 antibody MGC24293 antibody PRO1748 antibody SP-22 antibody PRDX3 antibody antioxidant protein 1 antibody peroxiredoxin-3 antibody "thioredoxin-dependent peroxide reductase mitochondrial antibo |
|---|---|
| Molecular Weight(MW) | 28 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
Peroxiredoxin 3 antibody [N1C3] detects Peroxiredoxin 3 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: Peroxiredoxin 3 protein stained by Peroxiredoxin 3 antibody [N1C3] diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded human lung cancer, using Peroxiredoxin 3 antibody at 1:100 dilution.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 C: Molt-4 D: Raji 12% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain , *PC3 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
