Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Arrestin 1 (Phospho-Ser412) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from COS7 cells treated with Etoposide using Arrestin 1 (Phospho-Ser412) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | Arrestin 1 (phospho Ser412) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARR1, arrestin 2, arrestin beta 1, beta-arrestin-1 |
| Product description | Functions in regulating agonist-mediated G-protein coupled receptor (GPCR) signaling by mediating both receptor desensitization and resensitization processes. During homologous desensitization, beta-arrestins bind to the GPRK-phosphorylated receptor and sterically preclude its coupling to the cognate G-protein; the binding appears to require additional receptor determinants exposed only in the active receptor conformation.1) Greg Buchanan. AACR Meeting Abstracts, Apr 2006; 2006: 430. |
| Immunogen | Arrestin 1 (phospho Ser412) antibody was raised against a peptide sequence around phosphorylation site of Serine 412 (T-G-S (p)-P-Q) derived from Human Arrestin 1. |
| Modification | p-Ser412 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARRB1 |
|---|---|
| Alternative Names | ARR1 arrestin 2 arrestin beta 1 beta-arrestin-1 |
| Molecular Weight(MW) | 50 kDa |
| Tissue Specificity | The antibody detects endogenous levels of Arrestin-1 only when phosphorylated at serine 412. |
| Entrez Gene | 408 |
|---|---|
| Protein Accession | NP_004032.2 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Arrestin 1 (Phospho-Ser412) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from COS7 cells treated with Etoposide using Arrestin 1 (Phospho-Ser412) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
