Application
Western blot analysis of ASAH1 in human heart tissue lysate with ASAH1 antibody at 1 μg/mL.Application
Immunofluorescence of ASAH1 in Human Heart cells with ASAH1 antibody at 20 μg/mL.Application
Immunohistochemistry of ASAH1 in human heart tissue with ASAH1 antibody at 2.5 μg/mL.| Product Name | ASAH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ASAH1 Antibody: AC, PHP, ASAH, PHP32, ACDase, SMAPME, HSD-33, HSD33, Acid ceramidase, Acylsphingosine deacylase, AC |
| Product description | ASAH1 Antibody: Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH1 is an acid ceramidase and key regulator of ceramide metabolism. Mutations in this gene results in Farber Lipogranulomatosis, a fatal human genetic disorder that results in the painful swelling of the joints and tendons and pulminary insufficiency, while a complete knockout of its expression is lethal in mice. Recent studies have shown elevated levels of ASAH1 in Alzheimer's disease (AD) patients correlating with a reduction in sphingomyelin and elevation of ceramide. Pretreatment of cultured neurons with recombinant AHAH1 prevented the cells from undergoing A-beta (Ab)-induced apoptosis.1) Nilsson A and Duan RD. Alkaline sphingomyelinases and ceramidases of the gastrointestinal tract. Chem. Phys. Lipids 1999; 102:97-105. |
| Immunogen | ASAH1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of the human ASAH1. The immunogen is located within amino acids 240 - 290 of ASAH1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ASAH1 antibody can be used for detection of ASAH1 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ASAH1 |
|---|---|
| Alternative Names | ASAH1 Antibody: AC PHP ASAH PHP32 ACDase SMAPME HSD-33 HSD33 Acid ceramidase Acylsphingosine deacylase AC |
| Molecular Weight(MW) | Predicted: 45 kDa Observed: 49 kDa |
| Sequence Similarities | 2) Cat. No. 10-501 - Human Heart Tissue Slide |
| Tissue Specificity | Multiple isoforms of ASAH1 are known to exist. |
Application
Western blot analysis of ASAH1 in human heart tissue lysate with ASAH1 antibody at 1 μg/mL.
Application
Immunofluorescence of ASAH1 in Human Heart cells with ASAH1 antibody at 20 μg/mL.
Application
Immunohistochemistry of ASAH1 in human heart tissue with ASAH1 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | ASAH1 antibody can be used for detection of ASAH1 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ASAH1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ASAH1 Antibody is supplied in PBS containing 0.02% sodium azide. |
