ASH2L is a component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. The protein may function as a transcriptional regulator and play a role in hematopoiesis.1) Daugherty, B.L. (2008) Cancer Res. 68 (3), 749-758.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ASH2L.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ASH2L antibody can be used for detection of ASH2L by ELISA at 1:1562500. ASH2L antibody can be used for detection of ASH2L by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in IHC on Human Placenta at 5.0 ug/ml.
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Testis at 5.0 ug/ml.
Positive Control
1) Cat. No. 1201 - HeLa Cell Lysate
Application Notes
ASH2L antibody can be used for detection of ASH2L by ELISA at 1:1562500. ASH2L antibody can be used for detection of ASH2L by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ASH2L antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.