Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1 (Ab-83). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using ASK1 (Ab-83).Application
Western blot analysis of lysed extracts from MDA-MB- 435 cells using ASK1 (Ab-83).| Product Name | ASK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ASK1, MEKK5, MAPKKK5, HER3, ASK-1, M3K5, MAP3K5, MAPK/ERK kinase kinase 5 |
| Product description | Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.1) Mabuchi S, et al. (2004) Endocrinology. 145 (1): 49-58. Yuan ZQ, et al. (2003) J Biol Chem. 278 (26): 23432-23440. Kim AH, et al. (2001) Mol Cell Biol. 21 (3): 893-901. |
| Immunogen | ASK1 (Ab-83) antibody was raised against a peptide sequence around aa.81~85 (G-S-S-V-G) derived from Human ASK1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP3K5 |
|---|---|
| Alternative Names | ASK1 MEKK5 MAPKKK5 HER3 ASK-1 M3K5 MAP3K5 MAPK/ERK kinase kinase 5 |
| Molecular Weight(MW) | 155 kDa |
| Tissue Specificity | This antibody detects endogenous level of total ASK1 protein. |
| Entrez Gene | 4217 |
|---|---|
| Protein Accession | NP_005914.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1 (Ab-83). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using ASK1 (Ab-83).
Application
Western blot analysis of lysed extracts from MDA-MB- 435 cells using ASK1 (Ab-83).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
