Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1 (Phospho-Ser966).Application
Western blot analysis of lysed extracts from various cells using ASK1 (Phospho-Ser966).| Product Name | ASK1 (phospho Ser966) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ASK1, MEKK5, MAPKKK5, SRK, ASK-1, M3K5, MAP3K5, MAPK/ERK kinase kinase 5 |
| Product description | Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.1) Zhang W, et al. (2005) J Biol Chem. 280 (19): 19036-19044. Fujii K, et al. (2004) Oncogene. 23 (29): 5099-5104. Goldman EH, et al. (2004) J Biol Chem 2004 Mar 12; 279 (11): 10442-10449. Zhang L, et al. (1999) Proc Natl Acad Sci U S A. 96 (15): 8511-8515. |
| Immunogen | ASK1 (Phospho-Ser966) antibody was raised against a peptide sequence around phosphorylation site of serine 966 (S-I-S (p) -L-P) derived from Human ASK1. |
| Modification | p-Ser966 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP3K5 |
|---|---|
| Alternative Names | ASK1 MEKK5 MAPKKK5 SRK ASK-1 M3K5 MAP3K5 MAPK/ERK kinase kinase 5 |
| Molecular Weight(MW) | 155 kDa |
| Tissue Specificity | This antibody detects endogenous level of ASK1 only when phosphorylated at serine 966. |
| Entrez Gene | 4217 |
|---|---|
| Protein Accession | NP_005914.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1 (Phospho-Ser966).
Application
Western blot analysis of lysed extracts from various cells using ASK1 (Phospho-Ser966).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
