Application
Immunocytochemistry of ASK1 in A431 cells with ASK1 antibody at 10 μg/mL.Application
Western blot analysis of ASK1 in SW1353 whole cell lysate with ASK1 antibody at 1:500 dilution.Application
Immunofluorescence of ASK1 in A431 cells with ASK1 antibody at 20 μg/mL.| Product Name | ASK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ASK1 Antibody: ASK1, MEKK5, MAPKKK5, ASK1, Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, ASK-1 |
| Product description | ASK1 Antibody: Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-α-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.1) Ichijo H, Nishida E, Irie K, ten Dijke P, Saitoh M, Moriguchi T, Takagi M, Matsumoto K, Miyazono K, Gotoh Y. Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 1997;275:90-4 |
| Immunogen | ASK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1. The sequence is different from that of mouse by last two amino acids. The immunogen is located within the last 50 amino acids of ASK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ASK1 antibody can be used for detection of ASK1 by Western blot at 0.5 μg/mL. A 155 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP3K5 |
|---|---|
| Alternative Names | ASK1 Antibody: ASK1 MEKK5 MAPKKK5 ASK1 Mitogen-activated protein kinase kinase kinase 5 Apoptosis signal-regulating kinase 1 ASK-1 |
| Molecular Weight(MW) | 155 kDa |
| Sequence Similarities | 2) Cat. No. 17-002 - A-431 Cell Slide |
Application
Immunocytochemistry of ASK1 in A431 cells with ASK1 antibody at 10 μg/mL.
Application
Western blot analysis of ASK1 in SW1353 whole cell lysate with ASK1 antibody at 1:500 dilution.
Application
Immunofluorescence of ASK1 in A431 cells with ASK1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1214 - SW1353 Cell Lysate |
|---|---|
| Application Notes | ASK1 antibody can be used for detection of ASK1 by Western blot at 0.5 μg/mL. A 155 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ASK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ASK1 Antibody is supplied in PBS containing 0.02% sodium azide. |
