Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using ATF-2 (Phospho-Ser472) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | ATF-2 (phospho Ser472) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activating 2, ATF2, cAMP response element binding protein CRE- BP1, CREB2, HB16 |
| Product description | Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.1) Maekawa T., EMBO J. 8:2023-2028(1989). |
| Immunogen | ATF-2 (phospho Ser472) antibody was raised against a peptide sequence around phosphorylation site of Serine 472 (E-A-V (p)-A-T) derived from Human ATF-2. |
| Modification | p-Ser472 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATF2 |
|---|---|
| Alternative Names | Activating 2 ATF2 cAMP response element binding protein CRE- BP1 CREB2 HB16 |
| Molecular Weight(MW) | 54 kDa |
| Tissue Specificity | The antibody detects endogenous levels of ATF-2 only when phosphorylated at serine 472. |
| Entrez Gene | 1386 |
|---|---|
| Protein Accession | NP_001243019.1 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using ATF-2 (Phospho-Ser472) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
