Application
Western blot analysis of lysed extracts from 293 and 3T3 cells using ATF2 (Ab-62 or 44).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2 (Ab-62 or 44). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using ATF2 (Ab-62 or 44).| Product Name | ATF2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HB16, CREB2, TREB7, CREB-2, CRE-BP1, JNK1, PRKM8, SAPK1, SAPK1C, CREBP1 |
| Product description | Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.1) Sevilla A, et al. (2004) J Biol Chem. 279 (26): 27458-27465. Sakurai A, et al. (1991) Biochem Biophys Res Commun. 181 (2): 629-635. Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089. Gupta S, et al. (1995) Science. 267: 389-393. Van Dam H, et al. (1995) EMBO J. 14 (8): 1798-1811. |
| Immunogen | ATF2 (Ab-62 or 44) antibody was raised against a peptide sequence around aa.serine 60~64 or 42~46 (N-D-S-V-I) derived from Human ATF2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATF2 |
|---|---|
| Alternative Names | HB16 CREB2 TREB7 CREB-2 CRE-BP1 JNK1 PRKM8 SAPK1 SAPK1C CREBP1 |
| Molecular Weight(MW) | 65 - 75 kDa |
| Tissue Specificity | This antibody detects endogenous level of total ATF-2 protein. |
| Entrez Gene | 1386 |
|---|---|
| Protein Accession | NP_001871.2 |
Application
Western blot analysis of lysed extracts from 293 and 3T3 cells using ATF2 (Ab-62 or 44).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2 (Ab-62 or 44). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using ATF2 (Ab-62 or 44).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
