Application
Western blot of human melanoma cells incubated with varying doses of the radiometric drug NCS showing phospho-specific immunolabeling of the ATF2 protein phosphorylated at Ser 490,498.| Product Name | ATF2 (phospho Ser 490 / 498) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HB16, CREB2, TREB7, CREB-2, CRE-BP1, CREBP1, Activating transcription factor 2, cAMP-dependent transcription factor ATF-2 |
| Product description | The activating transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Stress induced ATF-dependent transcription is dependent on phosphorylation of ATF. Serine 490 and serine 498 are novel phosphorylation sites on ATF that have recently been identified. In addition, ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered important substrates of signals upstream of the activation of genes associated with neuronal growth and differentiation. ATF expression has also been linked to the depression in humans.1) Laifenfeld, D., Karry, R., Grauer, E., Klein, E., and Ben-Shachar, D. "ATF2, a member of the CREB/ATF family of transcription factors, in chronicstress and consequent to antidepressant treatment: animal models and humanpost-mortem brains." Neuropsychopharmacology, 29 (2004) 589 - 97. |
| Immunogen | ATF-2 (Ser 490/498) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the human phospho Ser490/498 of ATF2. |
| Modification | p-Ser 490 / 498 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| The antibody also recognizes the phosphorylated 54k splice form of ATF2. Applications include Dot Blots (DB), Immunohistochemistry (IHC) and Western Blots (WB). When internally tested under ideal conditions the working dilutions were 1:1000 for DB, IHC and WB.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATF2 |
|---|---|
| Alternative Names | HB16 CREB2 TREB7 CREB-2 CRE-BP1 CREBP1 Activating transcription factor 2 cAMP-dependent transcription factor ATF-2 |
| Molecular Weight(MW) | 74 |
| Tissue Specificity | ATF2 antibody is specific for ~74k ATF2 phosphorylated at Ser490/498. |
Application
Western blot of human melanoma cells incubated with varying doses of the radiometric drug NCS showing phospho-specific immunolabeling of the ATF2 protein phosphorylated at Ser 490,498.| Application Notes | The antibody also recognizes the phosphorylated 54k splice form of ATF2. Applications include Dot Blots (DB), Immunohistochemistry (IHC) and Western Blots (WB). When internally tested under ideal conditions the working dilutions were 1:1000 for DB, IHC and WB.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
