Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2 (Phospho-Thr73 or 55). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using ATF2 (Phospho-Thr73 or 55).Application
Western blot analysis of lysed extracts from HepG2 cells untreated or treated with EGF using ATF2 (Phospho-Thr71 or 53).| Product Name | ATF2 (phospho Thr73 / 55) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HB16, CREB2, TREB7, CREB-2, CRE-BP1, APRF, CREBP1 |
| Product description | Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.1) Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101 (33): 12130-12135. Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089. Gupta S, et al. (1995) Science. 267: 389-393. Van Dam H, et al. (1995) EMBO J. 14 (8): 1798-1811. Livingstone C, et al. (1995) EMBO J. 14: 1785-1797. |
| Immunogen | ATF2 (Phospho-Thr73 or 55) antibody was raised against a peptide sequence around phosphorylation site of threonine 73 or 55 (T-P-T (p) -R-F) derived from Human ATF2. |
| Modification | p-Thr73 / 55 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATF2 |
|---|---|
| Alternative Names | HB16 CREB2 TREB7 CREB-2 CRE-BP1 APRF CREBP1 |
| Molecular Weight(MW) | 65 - 75 kDa |
| Tissue Specificity | This antibody detects endogenous level of ATF-2 only whenphosphorylated at threonine 73 or 55. |
| Entrez Gene | 1386 |
|---|---|
| Protein Accession | NP_001871.2 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2 (Phospho-Thr73 or 55). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using ATF2 (Phospho-Thr73 or 55).
Application
Western blot analysis of lysed extracts from HepG2 cells untreated or treated with EGF using ATF2 (Phospho-Thr71 or 53).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
