Application
Antibody used in WB on Human OVCAR-3 at 0.2-1 ug/ml.| Product Name | ATF3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATF3, |
| Product description | This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. ATF3 represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.1) Sampson, M.J., (2008) Eur. J. Endocrinol. 158 (5), 729-739. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATF3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ATF3 antibody can be used for detection of ATF3 by ELISA at 1:312500. ATF3 antibody can be used for detection of ATF3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATF3 |
|---|---|
| Alternative Names | ATF3 |
| Molecular Weight(MW) | 20 kDa |
Application
Antibody used in WB on Human OVCAR-3 at 0.2-1 ug/ml.| Positive Control | 1) OVCAR-3 Cell Lysate |
|---|---|
| Application Notes | ATF3 antibody can be used for detection of ATF3 by ELISA at 1:312500. ATF3 antibody can be used for detection of ATF3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ATF3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
