Overexpression of the bZIP protein ATF5, a transcriptional activator, stimulates asparagine synthetase promoter/reporter gene transcription via the nutrient-sensing response unit. ATF5 is also a new interacting partner of cyclin D3.1) Zhou, D., (2008) J. Biol. Chem. 283 (11), 7064-7073.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATF5.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ATF5 antibody can be used for detection of ATF5 by ELISA at 1:1562500. ATF5 antibody can be used for detection of ATF5 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Liver.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
ATF5 antibody can be used for detection of ATF5 by ELISA at 1:1562500. ATF5 antibody can be used for detection of ATF5 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ATF5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.