Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).Application
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: human heart lysate Lane 4: mouse liver lysate Lysates/proteins at 20 ug per lane.| Product Name | ATF5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cyclic AMP-dependent transcription factor ATF-5, cAMP-dependent transcription factor ATF-5, Activating transcription factor 5, Transcription factor ATFx, ATF5, ATFX |
| Product description | Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4.1) Wei, Y., et al. J. Biochem. 148(2):171-178(2010) |
| Immunogen | This ATF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-216 amino acids from the C-terminal region of human ATF5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATF5 |
|---|---|
| Alternative Names | Cyclic AMP-dependent transcription factor ATF-5 cAMP-dependent transcription factor ATF-5 Activating transcription factor 5 Transcription factor ATFx ATF5 ATFX |
| Molecular Weight(MW) | 31 kDa |
Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Application
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: human heart lysate Lane 4: mouse liver lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
