Application
Western blot analysis of ATF6 in MCF7 cell lysate with ATF6 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunocytochemistry of ATF6 in A20 with ATF6 antibody at 10 μg/mL.| Product Name | ATF6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATF6 Antibody: ATF6A, Cyclic AMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, cAMP-dependent transcription factor ATF-6 alpha |
| Product description | ATF6 Antibody: Disruptions of protein folding and maturation in the endoplasmic reticulum (ER) result in the activation of the unfolded protein response (UPR), an integrated cellular signaling pathway that transmits information from the ER lumen to the cytoplasm and nucleus. Activating transcription factor 6 (ATF6) as well as the ER-transmembrane protein kinases IRE1p and PERK are the major transducers of the UPR. ATF6 is an ER transmembrane protein that is normally bound to the ER chaperone GRP78, but upon ER stress is released from GRP78 and proteolytically cleaved to yield a cytosolic fragment which then migrates to the nucleus, and together with the transcription factor XBP-1, activates transcription of UPR-responsive genes. ATF6 has two isoforms (ATF6α and ATF6β); only ATF6α is recognized by this antibody.1) Liu CY and Kaufman RJ. The unfolded protein response. J. Cell Sci. 2003; 1861-2. |
| Immunogen | ATF6 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ATF6. The immunogen is located within amino acids 30 - 80 of ATF6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC |
| ATF6 antibody can be used for the detection of ATF6 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATF6 |
|---|---|
| Alternative Names | ATF6 Antibody: ATF6A Cyclic AMP-dependent transcription factor ATF-6 alpha Activating transcription factor 6 alpha cAMP-dependent transcription factor ATF-6 alpha |
| Sequence Similarities | 3) Cat. No. 17-208 - A-20 Cell Slide |
Application
Western blot analysis of ATF6 in MCF7 cell lysate with ATF6 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunocytochemistry of ATF6 in A20 with ATF6 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | ATF6 antibody can be used for the detection of ATF6 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ATF6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ATF6 Antibody is supplied in PBS containing 0.02% sodium azide. |
