Application
Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 μg/mL.Application
Immunohistochemistry of ATF6 in human pancreas tissue with ATF6 antibodyApplication
Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 μg/ml.Application
Immunohistochemistry of ATF6 in human breast tissue with ATF6 antibody at 5 μg/ml.Application
Immunofluorescence of ATF6 in human breast tissue with ATF6 antibodyApplication
Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody| Product Name | ATF6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATF6 Antibody: ATF6A, Cyclic AMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, cAMP-dependent transcription factor ATF-6 alpha |
| Product description | ATF6 Antibody: Disruptions of protein folding and maturation in the endoplasmic reticulum (ER) result in the activation of the unfolded protein response (UPR), an integrated cellular signaling pathway that transmits information from the ER lumen to the cytoplasm and nucleus. Activating transcription factor 6 (ATF6) as well as the ER-transmembrane protein kinases IRE1p and PERK are the major transducers of the UPR. ATF6 is an ER transmembrane protein that is normally bound to the ER chaperone GRP78, but upon ER stress is released from GRP78 and proteolytically cleaved to yield a cytosolic fragment which then migrates to the nucleus, and together with the transcription factor XBP-1, activates transcription of UPR-responsive genes. ATF6 has two isoforms (ATF6α and ATF6β); only ATF6α is recognized by this antibody.1) Liu CY and Kaufman RJ. The unfolded protein response. J. Cell Sci. 2003; 1861-2. |
| Immunogen | ATF6 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ATF6. The immunogen is located within amino acids 470 - 520 of ATF6. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFIHC-PWB |
| WB:1:500~1:2000 ICC/IF:1:100~1:500 IHC-p:1:50~1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATF6 |
|---|---|
| Alternative Names | ATF6 Antibody: ATF6A Cyclic AMP-dependent transcription factor ATF-6 alpha Activating transcription factor 6 alpha cAMP-dependent transcription factor ATF-6 alpha |
| Sequence Similarities | 2) Cat. No. 17-012 - MCF7 Cell Slide |
Application
Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 μg/mL.
Application
Immunohistochemistry of ATF6 in human pancreas tissue with ATF6 antibody
Application
Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 μg/ml.
Application
Immunohistochemistry of ATF6 in human breast tissue with ATF6 antibody at 5 μg/ml.
Application
Immunofluorescence of ATF6 in human breast tissue with ATF6 antibody
Application
Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 ICC/IF:1:100~1:500 IHC-p:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | ATF6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ATF6 Antibody is supplied in PBS containing 0.02% sodium azide. |
