Application
Immunohistochemistry of ATG12 in human brain tissue with ATG12 antibody at 2.5 μg/mL.Application
Immunofluorescence of ATG12 in Human Brain cells with ATG12 antibody at 20 μg/mL.Application
Western blot analysis of ATG12 in mouse heart tissue lysate with ATG12 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | ATG12 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATG12 Antibody: APG12, FBR93, APG12L, HAPG12, APG12, Ubiquitin-like protein ATG12, Autophagy-related protein 12, APG12-like |
| Product description | ATG12 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene2004; 23:2891-906. |
| Immunogen | ATG12 antibody was raised against a 15 amino acid synthetic peptide from near the center of human ATG12. The immunogen is located within the first 50 amino acids of ATG12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ATG12 antibody can be used for the detection of ATG10 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATG12 |
|---|---|
| Alternative Names | ATG12 Antibody: APG12 FBR93 APG12L HAPG12 APG12 Ubiquitin-like protein ATG12 Autophagy-related protein 12 APG12-like |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of ATG12 in human brain tissue with ATG12 antibody at 2.5 μg/mL.
Application
Immunofluorescence of ATG12 in Human Brain cells with ATG12 antibody at 20 μg/mL.
Application
Western blot analysis of ATG12 in mouse heart tissue lysate with ATG12 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1401 - Mouse Heart Tissue Lysate |
|---|---|
| Application Notes | ATG12 antibody can be used for the detection of ATG10 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ATG12 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ATG12 Antibody is supplied in PBS containing 0.02% sodium azide. |
