Application
Immunohistochemistry of ATG13 in mouse heart with ATG13 antibody at 5 μg/mL.Application
Immunofluorescence of ATG13 in Mouse Heart tissue with ATG13 antibody at 20 μg/mL.Application
Western blot analysis of ATG13 in rat heart tissue lysate with ATG13 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | ATG13 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATG13 Antibody: KIAA0652, PARATARG8, KIAA0652, Autophagy-related protein 13 |
| Product description | ATG13 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG13 forms a complex with ULK1 and ULK2, the mammalian homologs of ATG1, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. Knockdown of ATG13 inhibits autophagosome formation.1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906. |
| Immunogen | ATG13 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ATG13. The immunogen is located within amino acids 450 - 500 of ATG13. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHC-P |
| ATG13 antibody can be used for detection of ATG13 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATG13 |
|---|---|
| Alternative Names | ATG13 Antibody: KIAA0652 PARATARG8 KIAA0652 Autophagy-related protein 13 |
| Molecular Weight(MW) | Predicted: 44, 53, 57, 61 kDa Observed: 49 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| Tissue Specificity | Multiple isoforms of ATG13 are known to exist. |
| Entrez Gene | 9776 |
|---|---|
| Protein Accession | NP_001136145 |
Application
Immunohistochemistry of ATG13 in mouse heart with ATG13 antibody at 5 μg/mL.
Application
Immunofluorescence of ATG13 in Mouse Heart tissue with ATG13 antibody at 20 μg/mL.
Application
Western blot analysis of ATG13 in rat heart tissue lysate with ATG13 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1461 - Rat Heart Tissue Lysate |
|---|---|
| Application Notes | ATG13 antibody can be used for detection of ATG13 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ATG13 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ATG13 Antibody is supplied in PBS containing 0.02% sodium azide. |
