Application
Immunofluorescence of ATG3 in human kidney with ATG3 antibody at 20 μg/mL.Application
Immunohistochemistry of ATG3 in human kidney tissue with ATG3 antibody at 5 μg/mL.Application
Western blot analysis of ATG3 in Mouse kidney tissue Lysate with ATG3 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | ATG3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATG3 Antibody: APG3, APG3L, PC3-96, APG3-LIKE, APG3, Ubiquitin-like-conjugating enzyme ATG3, Autophagy-related protein 3, APG3-like |
| Product description | ATG3 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG3 (APG3) is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. It functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, which is critical for autophagy.1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene2004; 23:2891-906. |
| Immunogen | ATG3 antibody was raised against an 18 amino acid synthetic peptide near the center of human ATG3. The immunogen is located within amino acids 180 - 230 of ATG3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ATG3 antibody can be used for detection of ATG3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATG3 |
|---|---|
| Alternative Names | ATG3 Antibody: APG3 APG3L PC3-96 APG3-LIKE APG3 Ubiquitin-like-conjugating enzyme ATG3 Autophagy-related protein 3 APG3-like |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
Application
Immunofluorescence of ATG3 in human kidney with ATG3 antibody at 20 μg/mL.
Application
Immunohistochemistry of ATG3 in human kidney tissue with ATG3 antibody at 5 μg/mL.
Application
Western blot analysis of ATG3 in Mouse kidney tissue Lysate with ATG3 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | ATG3 antibody can be used for detection of ATG3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ATG3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ATG3 Antibody is supplied in PBS containing 0.02% sodium azide. |
