Application
Immunofluorescence of ATG4B in human spleen tissue with ATG4B antibody at 20 μg/ml.Application
Immunohistochemistry of ATG4B in human spleen tissue with ATG4B antibody at 5 μg/ml.Application
Western blot analysis of ATG4B in (A) Ramos and (B) Jurkat cell lysate with ATG4B antibody at 1 μg/mL.| Product Name | ATG4B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Autophagy related 4B cysteine peptidase, APG4B, APG4-B, AUTL1 |
| Product description | Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4B, also known as AUTL1, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3). ATG4 is required for ATG8 conjugation to phosphatidylethanolamine on autophagosomal membranes. In mammals, each ATG4 homolog shows a selective preference for the ATG8 homologs (4). ATG4B has been found to be a novel protective protein in inflammatory colitis (5).1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906. |
| Immunogen | ATG4B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ATG4B. The immunogen is located within the last 50 amino acids of ATG4B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ATG4B antibody can be used for detection of ATG4B by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATG4B |
|---|---|
| Alternative Names | Autophagy related 4B cysteine peptidase APG4B APG4-B AUTL1 |
| Molecular Weight(MW) | Predicted: 43 kDa Observed: 46 kDa |
| Sequence Similarities | 4) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | ATG4B antibody is human, mouse and rat reactive. At least two isoforms of ATG4B are known to exist; this antibody will detect only the larger isoform. ATG4B is predicted to not cross-react with other ATG4 proteins. |
Application
Immunofluorescence of ATG4B in human spleen tissue with ATG4B antibody at 20 μg/ml.
Application
Immunohistochemistry of ATG4B in human spleen tissue with ATG4B antibody at 5 μg/ml.
Application
Western blot analysis of ATG4B in (A) Ramos and (B) Jurkat cell lysate with ATG4B antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1225 - Ramos Cell Lysate |
|---|---|
| Application Notes | ATG4B antibody can be used for detection of ATG4B by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ATG4B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ATG4B antibody is supplied in PBS containing 0.02% sodium azide. |
