Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATM (Phospho-Ser1981).Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using ATM (Phospho-Ser1981).| Product Name | ATM (phospho Ser1981) Antibody |
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| Antibody Type | Primary Antibodies |
| Product description | ATM encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene.1) Gupta A. et al. (2005) Mol Cell Biol. 25 (12): 5292-5305. Bernstein JL. et al. (2002) Breast Cancer Res. 4 (6): 249-252. Silverman J. et al. (2004) Genes Dev. 18 (17): 2108-2119. Nakada D. et al. (2003) Nucleic Acids Res. 31 (6): 1715-1724. |
| Immunogen | ATM (Phospho-Ser1981) antibody was raised against a peptide sequence around phosphorylation site of serine 1981 (E-G-S (p) -Q-S) derived from Human ATM. |
| Modification | p-Ser1981 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC,WB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | Human,Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATM |
|---|---|
| Alternative Names | AT1 ATA ATC ATD ATE ATDC TEL1 TELO1 APRF Ataxia telangiectasia mutated homolog Ataxia telangiectasia mutated kinase ATM |
| Molecular Weight(MW) | 350 kDa |
| Tissue Specificity | This antibody detects endogenous level of ATM only when phosphorylated at serine 1981. |
| Entrez Gene | 472 |
|---|---|
| Protein Accession | NP_000042.3 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATM (Phospho-Ser1981).
Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using ATM (Phospho-Ser1981).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
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| Form | Liquid |
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| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
