Application
Immunohistochemistry of ATOH8 in mouse brain tissue with ATOH8 antibody at 5 μg/mL.Application
Western blot analysis of ATOH8 in A-20 cell lysate with ATOH8 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | ATOH8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATOH8 Antibody: HATH6, bHLHa21, ATH6, BHLHA21, Protein atonal homolog 8, Class A basic helix-loop-helix protein 21 |
| Product description | ATOH8 Antibody: Basic helix-loop-helix (bHLH) transcription factors play important roles in differentiation processes during embryonic development of vertebrates. ATOH8 (MATH6) is a tissue-restricted member of the atonal superfamily of bHLH transcription factors that exhibits 43-57% identity in the bHLH domain with other mammalian atonal paralogs including the NeuroD and Neurogenin factors. In the mouse, ATOH8 has been implicated in the specification and differentiation of neuronal cell lineages in the brain and may also participate in kidney development. Recent studies show that ATOH8 is a novel component of the pancreatic transcriptional network during embryonic development and suggest a potential role as a modulator of the differentiation program initiated by the pro-endocrine factor Neurog3. It is indispensable for early embryonic development, suggesting a more widespread function for this factor in tissue-specific differentiation processes that are dependent on class II bHLH genes.1) Wasserman SM, Mehraban F, Komuves LG, et al. Gene expression profile of human endothelial cells exposed to sustained fluid shear stress. Physiol. Genomics 2002;12:13-23. |
| Immunogen | ATOH8 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ATOH8. The immunogen is located within the last 50 amino acids of ATOH8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| ATOH8 antibody can be used for detection of ATOH8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATOH8 |
|---|---|
| Alternative Names | ATOH8 Antibody: HATH6 bHLHa21 ATH6 BHLHA21 Protein atonal homolog 8 Class A basic helix-loop-helix protein 21 |
| Molecular Weight(MW) | Predicted: 35 kDa Observed: 40 kDa |
Application
Immunohistochemistry of ATOH8 in mouse brain tissue with ATOH8 antibody at 5 μg/mL.
Application
Western blot analysis of ATOH8 in A-20 cell lysate with ATOH8 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | ATOH8 antibody can be used for detection of ATOH8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ATOH8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ATOH8 Antibody is supplied in PBS containing 0.02% sodium azide. |
