Application
Antibody used in WB on Human HepG2 cells at 0.25 ug/ml.Application
Antibody used in IHC on Human Heart.Application
Antibody used in IHC.| Product Name | ATP1B1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATP1B1, ATP1B, MGC1798 |
| Product description | ATP1B1 belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit.The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been identified.1) Vagin, O., (2005) J. Biol. Chem. 280 (52), 43159-43167. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATP1B1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| ATP1B1 antibody can be used for detection of ATP1B1 by ELISA at 1:62500. ATP1B1 antibody can be used for detection of ATP1B1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP1B1 |
|---|---|
| Alternative Names | ATP1B1 ATP1B MGC1798 |
| Molecular Weight(MW) | 35 kDa, 35 kDa |
| Entrez Gene | 481 |
|---|---|
| Protein Accession | NP_001001787 |
Application
Antibody used in WB on Human HepG2 cells at 0.25 ug/ml.
Application
Antibody used in IHC on Human Heart.
Application
Antibody used in IHC.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | ATP1B1 antibody can be used for detection of ATP1B1 by ELISA at 1:62500. ATP1B1 antibody can be used for detection of ATP1B1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ATP1B1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
