Application
Western blot analysis of ATP6V1A Antibody in A2058 cell line lysates (35ug/lane)Application
ATP6V1A Antibody IHC analysis in formalin fixed and paraffin em| Product Name | ATP6V1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues.1) Martins-de-Souza, D., et al.Psychiatr Res 43(11):978-986(2009) |
| Immunogen | This ATP6V1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 441-468 amino acids from the Central region of human ATP6V1A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-P,WB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | Human,Monkey,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP6V1A |
|---|---|
| Alternative Names | V-type proton ATPase catalytic subunit A V-ATPase subunit A V-ATPase 69 kDa subunit Vacuolar ATPase isoform VA68 Vacuolar proton pump subunit alpha ATP6V1A ATP6A1 ATP6V1A1 VPP2 |
| Molecular Weight(MW) | 68 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of ATP6V1A Antibody in A2058 cell line lysates (35ug/lane)
Application
ATP6V1A Antibody IHC analysis in formalin fixed and paraffin em| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
