Application
Western Blot at 1:2000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Confocal immunofluorescent analysis of ATXN1 Antibody (S776) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | ATXN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ataxin-1, Spinocerebellar ataxia type 1 protein, ATXN1, ATX1, SCA1 |
| Product description | The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. The function of the ataxins is not known.1) Hong,S., Biochem. Biophys. Res. Commun. 371 (2), 256-260 (2008) |
| Immunogen | This ATXN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 754-781 amino acids from human ATXN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:2000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATXN1 |
|---|---|
| Alternative Names | Ataxin-1 Spinocerebellar ataxia type 1 protein ATXN1 ATX1 SCA1 |
| Molecular Weight(MW) | 87 kDa |
Application
Western Blot at 1:2000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Confocal immunofluorescent analysis of ATXN1 Antibody (S776) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | For WB starting dilution is: 1:2000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
