Application
Western blot analysis of extracts from COS7 cells treated with Nocodazole using Phospho-AurB (Thr232) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | AurB (phospho Thr232) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AIK2, AIM-1, ARK2, AURKB, Aurora-B |
| Product description | Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity.1) Caldas GV, DeLuca KF, DeLuca JG (2013)J Cell Biol 203, 957-69. Zhang L, et al. (2012)J Biol Chem 287, 34069-77. Ratushny V, et al. (2012)Oncogene 31, 1217-27 . |
| Immunogen | AurB (phospho Thr232) antibody was raised against a peptide sequence around phosphorylation site of threonine 232 (R-K-T (p)-M-C) derived from Human AurB. |
| Modification | p-Thr232 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AURKB |
|---|---|
| Alternative Names | AIK2 AIM-1 ARK2 AURKB Aurora-B |
| Molecular Weight(MW) | 39 kDa |
| Tissue Specificity | The antibody detects endogenous level of AurB only when phosphorylated at threonine 232. |
| Entrez Gene | 9212 |
|---|---|
| Protein Accession | NP_001243763.1 |
Application
Western blot analysis of extracts from COS7 cells treated with Nocodazole using Phospho-AurB (Thr232) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
