Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of AURKB using rabbit polyclonal Aurora-B (ARK/STK12) Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AURKB gene (Lane 2).Application
Antibody is used in Western blot to detect STK12 in mouse spleen tissue lysate.| Product Name | Aurora-B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Aurora kinase B, Aurora 1, Aurora- and IPL1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, ARK-2, Aurora-related kinase 2, STK-1, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase 5, Serine/threonine-protein kinas |
| Product description | Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. STK12 (Aurora kinase B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A localizes to centrosomes1) Kimura, M., et al., Biochem. Biophys. Res. Commun. 316(3):930-936 (2004). |
| Immunogen | This Aurora-B (ARK/STK12) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-313 amino acids from the Central region of human Aurora-B (ARK/STK12). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | AURKB |
|---|---|
| Alternative Names | Aurora kinase B Aurora 1 Aurora- and IPL1-like midbody-associated protein 1 AIM-1 Aurora/IPL1-related kinase 2 ARK-2 Aurora-related kinase 2 STK-1 Serine/threonine-protein kinase 12 Serine/threonine-protein kinase 5 Serine/threonine-protein kinas |
| Molecular Weight(MW) | 39 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of AURKB using rabbit polyclonal Aurora-B (ARK/STK12) Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AURKB gene (Lane 2).
Application
Antibody is used in Western blot to detect STK12 in mouse spleen tissue lysate.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
