Application
Western Blot analysis of PSCD4 expression in transfected 293T cell line by PSCD4 polyclonal antibody. A: Non-transfected lysate. B: PSCD4 transfected lysate. 12% SDS PAGE diluted at 1:500| Product Name | PSCD4 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PSCD4 (pleckstrin homology, Sec7 and coiled-coil domains 4)The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The encoded protein exhibits GEP activity in vitro with both ARF1 and ARF5 but is inactive with ARF6. The structures of this gene and CYTH1 are very similar. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human PSCD4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CYT4 antibody DJ63G5.1 antibody PSCD4 antibody CYTH4 antibody "pleckstrin homology Sec7 and coiled-coil domains 4 antibody" cytohesin-4 antibody "pleckstrin homology Sec7 and coiled/coil domains 4 antibody" "PH SEC7 and coiled-coil domain-contain |
|---|---|
| Target | PSCD4 |
| Molecular Weight(MW) | 46 kDa (note) |
Application
Western Blot analysis of PSCD4 expression in transfected 293T cell line by PSCD4 polyclonal antibody. A: Non-transfected lysate. B: PSCD4 transfected lysate. 12% SDS PAGE diluted at 1:500| Positive Control | PSCD4 tansfected 293T cell |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
