Application
Immunohistochemistry of AXIN2 in rat lung tissue with AXIN2 antibody at 5 μg/mL.Application
Immunofluorescence of AXIN2 in Rat Lung cells with AXIN2 antibody at 20 μg/mL.Application
Immunohistochemistry of AXIN2 in mouse lung tissue with AXIN2 antibody at 5 μg/mL.Application
Western blot analysis of AXIN2 in mouse lung lysate with AXIN2 antibody at 1 μg/mL.| Product Name | AXIN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | AXIN2 Antibody: Like the related protein AXIN1, AXIN2 is thought to play an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway. In mouse, AXIN2 organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and AXIN2, which leads to the degradation of beta-catenin, which is thought to be an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.1) Katoh M and Katoh M. WNT signaling pathway and stem cell signaling network. Clin. Cancer Res.2007; 13:4042-5. |
| Immunogen | AXIN2 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human AXIN2. The immunogen is located within amino acids 780 - 830 of AXIN2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| AXIN2 antibody can be used for detection of AXIN2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AXIN2 |
|---|---|
| Alternative Names | AXIN2 Antibody: AXIL ODCRCS Axin-2 Axin-like protein Axil |
| Molecular Weight(MW) | 85, 93 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (85%) |
| Tissue Specificity | AXIN2 antibody is predicted to not cross-react with AXIN1. At least four isoforms of AXIN2 are known to exist; this antibody will recognize all four. |
Application
Immunohistochemistry of AXIN2 in rat lung tissue with AXIN2 antibody at 5 μg/mL.
Application
Immunofluorescence of AXIN2 in Rat Lung cells with AXIN2 antibody at 20 μg/mL.
Application
Immunohistochemistry of AXIN2 in mouse lung tissue with AXIN2 antibody at 5 μg/mL.
Application
Western blot analysis of AXIN2 in mouse lung lysate with AXIN2 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1402 - Mouse Lung Tissue Lysate |
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| Application Notes | AXIN2 antibody can be used for detection of AXIN2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | AXIN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | AXIN2 Antibody is supplied in PBS containing 0.02% sodium azide. |
