Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using B-Raf (Phospho-Thr598) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | B-RAF (phospho Thr599) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B-RAF, RAFB1, RMIL, BRAF1, c-RMIL |
| Product description | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.1) Armelle Calipel, J. Biol. Chem., Oct 2003; 278: 42409 - 42418. |
| Immunogen | B-RAF (phospho Thr599) antibody was raised against a peptide sequence around phosphorylation site of threonine 599 (L-A-T (p)-V-K) derived from Human B-RAF. |
| Modification | p-Thr599 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BRAF |
|---|---|
| Alternative Names | B-RAF RAFB1 RMIL BRAF1 c-RMIL |
| Molecular Weight(MW) | 84 kDa |
| Tissue Specificity | The antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 598. |
| Entrez Gene | 673 |
|---|---|
| Protein Accession | NP_004324.2 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using B-Raf (Phospho-Thr598) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
