Application
Western blot analysis of extracts from K562 cells treated with EGF using B-Raf (Phospho-Thr753) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | B-Raf (phospho Thr753) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B-RAF, RAFB1, RMIL, p94 |
| Product description | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.1) Stephens R.M., Mol. Cell. Biol. 12:3733-3742(1992). |
| Immunogen | B-Raf (phospho Thr753) antibody was raised against a peptide sequence around phosphorylation site of threonine 753 (P-K-T (p)-P-L) derived from Human B-Raf. |
| Modification | p-Thr753 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BRAF |
|---|---|
| Alternative Names | B-RAF RAFB1 RMIL p94 |
| Molecular Weight(MW) | 84 kDa |
| Tissue Specificity | The antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 753 |
| Entrez Gene | 673 |
|---|---|
| Protein Accession | NP_004324.2 |
Application
Western blot analysis of extracts from K562 cells treated with EGF using B-Raf (Phospho-Thr753) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
