Application
Immunohistochemistry of B-raf in human small intestine tissue with B-raf antibody at 2.5 μg/ml.Application
Western blot analysis of B-raf in human brain tissue lysate with B-raf antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of B-raf in human small intestine tissue with B-raf antibody at 20 μg/ml.| Product Name | B-raf Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | B-raf Antibody: B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance.1) McCubrey JA, Steelman LS, Chappell WH, et al. Roles of the RAF/MEK/ERK pathway in cell growth, malignant transformation and drug resistance. Biochim. Biophys. Acta 2007; 1773:1263-84. |
| Immunogen | B-raf antibody was raised against a 19 amino acid peptide near the center of human B-raf. The immunogen is located within amino acids 330 - 380 of B-raf. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA,IF,IHC-P,WB |
| B-raf antibody can be used for detection of B-raf by Western blot at 1 and 2 μg/mL. | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BRAF |
|---|---|
| Alternative Names | B-raf Antibody: NS7 BRAF1 RAFB1 B-RAF1 Proto-oncogene B-Raf |
| Molecular Weight(MW) | Predicted: 84 Observed: 99, multiple post-translational modifications, commonly observed at 95-100kDa |
| Sequence Similarities | 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| Tissue Specificity | At least two isoforms of B-raf are known to exist; this antibody will detect both isoforms. This antibody will not cross-react with C-raf. |
Application
Immunohistochemistry of B-raf in human small intestine tissue with B-raf antibody at 2.5 μg/ml.
Application
Western blot analysis of B-raf in human brain tissue lysate with B-raf antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of B-raf in human small intestine tissue with B-raf antibody at 20 μg/ml.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | B-raf antibody can be used for detection of B-raf by Western blot at 1 and 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | B-raf antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | B-raf Antibody is supplied in PBS containing 0.02% sodium azide. |
