Application
Flow cytometric analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of B3GALT6 Antibody in Ramos cell line lysates (35ug/lane)Application
B3GALT6 Antibody in Ramos cell line lysates (35ug/lane).B3GALT6 (arrow) ) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | B3GALT6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Beta-1,3-galactosyltransferase 6, Beta-1,3-GalTase 6, Beta3Gal-T6, Beta3GalT6, GAG GalTII, Galactosyltransferase II, Galactosylxylosylprotein 3-beta-galactosyltransferase, UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 6, B3GALT6 |
| Product description | B3GALT6, Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-linked galactose residue. B3GALT6 has a preference for galactose-beta-1,4-xylose that is found in the linker region of glycosaminoglycans, such as heparan sulfate and chondroitin sulfate. B3GALT6 has no activity towards substrates with terminal glucosamine or galactosamine residues.1) Bai, X., et al. J. Biol. Chem. 276(51):48189-48195(2001) Zhou, D., et al. Proc. Natl. Acad. Sci. U.S.A. 96(2):406-411(1999) |
| Immunogen | This B3GALT6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-269 amino acids from the C-terminal region of human B3GALT6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | B3GALT6 |
|---|---|
| Alternative Names | Beta-1 3-galactosyltransferase 6 Beta-1 3-GalTase 6 Beta3Gal-T6 Beta3GalT6 GAG GalTII Galactosyltransferase II Galactosylxylosylprotein 3-beta-galactosyltransferase UDP-Gal:betaGal beta 1 3-galactosyltransferase polypeptide 6 B3GALT6 |
| Molecular Weight(MW) | 37 kDa |
Application
Flow cytometric analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of B3GALT6 Antibody in Ramos cell line lysates (35ug/lane)
Application
B3GALT6 Antibody in Ramos cell line lysates (35ug/lane).B3GALT6 (arrow) ) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
