Application
Western Blot at 1:2000 dilution Lane 1: human liver lysate Lane 2: HepG2 whole cell lysate Lane 3: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | BAAT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Involved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs.1) Falany C.N.,et al.J. Biol. Chem. 269:19375-19379(1994). |
| Immunogen | This BAAT antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 107-148 amino acids from human BAAT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BAAT |
|---|---|
| Alternative Names | Bile acid-CoA:amino acid N-acyltransferase BACAT BAT 2.3.1.65 Glycine N-choloyltransferase Long-chain fatty-acyl-CoA hydrolase 3.1.2.2 BAAT |
| Molecular Weight(MW) | 46 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: human liver lysate Lane 2: HepG2 whole cell lysate Lane 3: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
