Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser136).Application
Western blot analysis of lysed extracts from HT29 cells untreated or treated with UV using BAD (Phospho-Ser136).| Product Name | BAD (phospho Ser136) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.1) Wang XQ, et al. (2001) . J Biol Chem. 276 (48): 44504-44511. Lee YI, et al. (2001) . J Biol Chem. 276 (20): 16969-16977. Maiti D, et al. (2001) . J Biol Chem. 276 (1): 329-333. |
| Immunogen | BAD (Phospho-Ser136) antibody was raised against a peptide sequence around phosphorylation site of serine136 (S-R-S (p) -A-P) derived from Mouse BAD. |
| Modification | p-Ser136 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BAD |
|---|---|
| Alternative Names | BBC2 BCL2L8 BBC6 |
| Molecular Weight(MW) | 23 kDa |
| Tissue Specificity | This antibody detects endogenous level of BAD only when phosphorylated at serine136. |
| Entrez Gene | 572 |
|---|---|
| Protein Accession | NP_004313.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser136).
Application
Western blot analysis of lysed extracts from HT29 cells untreated or treated with UV using BAD (Phospho-Ser136).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
