Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser91/128) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from COS7 cells treated with TNF-a using BAD (Phospho-Ser91/128) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | BAD (phospho Ser91/128) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.1) Wang H.-G., Cell 87:629-638(1996). |
| Immunogen | BAD (phospho Ser91/128) antibody was raised against a peptide sequence around phosphorylation site of Serine 91 (E-P-S (p)-P-F) derived from Human BAD or Serine 128 (E-P-S (p)-P-F) derived from Mouse BAD. |
| Modification | p-Ser91/128 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BAD |
|---|---|
| Alternative Names | BAD BBC6 BCL2L8 Bcl- XL/Bcl-2 associated death promoter |
| Molecular Weight(MW) | 22 kDa |
| Tissue Specificity | The antibody detects endogenous levels of BAD only when phosphorylated at serine 91/ serine 128. |
| Entrez Gene | 572 |
|---|---|
| Protein Accession | NP_004313.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser91/128) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from COS7 cells treated with TNF-a using BAD (Phospho-Ser91/128) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
