Application
Immunohistochemistry staining of BAD in cerebellum tissue using BAD Antibody.| Product Name | BAD Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax. Bad activity is regulated by its phosphorylation; it is inactivated by kinases such as Akt and MAP kinase and thus promotes cell survival, whereas JNK-induced phosphorylation promotes the apoptotic role of Bad. |
| Immunogen | BAD antibody was raised against amino acids surrounding Serine 112 of BAD (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| BAD antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 5 μg/mL, and immunofluorescence starting at 10 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BAD |
|---|---|
| Alternative Names | BAD BBC2 Bcl-2-like protein 8 BCL2-binding component 6 BCL2L8 BBC6 BCL-X/BCL-2 binding protein Bcl-2-binding component 6 Bcl2 antagonist of cell death BCL2-binding protein Bcl2-L-8 |
| Tissue Specificity | pSer112 |
Application
Immunohistochemistry staining of BAD in cerebellum tissue using BAD Antibody.| Application Notes | BAD antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 5 μg/mL, and immunofluorescence starting at 10 μg/mL. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | BAD antibody should be stored long term (months) at -80 ˚C and short term (days) at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.02% sodium azide, 50% glycerol. |
